Exogenous papain supplementation: impacts on growth, digestibility, digestive enzyme activities and oxidative stress in Labeo rohita fingerlings

Table 1 Ingredients proportion and proximate composition of experimental diet supplemented with different proportion of papain enzyme during experimental period

S. No	Ingredients	Quantity (g/Kg)
Control diet
1	GNOC ^a	400
2	Rice bran ^a	400
3	Wheat flour ^a	190
4	Vitamin-mineral mixture ^b	10
Experimental diets
		T0	T1	T2	T3	T4	T5
1	Control diets (g)	1000	990	980	970	960	950
2	Papain enzyme ^c (g)	0	10	20	30	40	50
Proximate composition of the diet (on dry matter basis; mean of triplicates)
1	Moisture (%)	7.13 ± 0.12	7.27 ± 0.09	7.37 ± 0.03	7.43 ± 0.12	7.63 ± 0.03	7.70 ± 0.06
2	Crude protein (%)	22.71 ± 0.02	23.00 ± 0.29	23.32 ± 0.21	23.10 ± 0.17	22.90 ± 0.29	22.80 ± 0.29
3	Crude lipid (%)	5.09 ± 0.15	5.10 ± 0.06	5.12 ± 0.09	5.14 ± 0.02	5.18 ± 0.01	5.19 ± 0.01
4	Total ash (%)	6.53 ± 0.01	6.52 ± 0.03	6.52 ± 0.00	6.51 ± 0.01	6.51 ± 0.00	6.51 ± 0.00
5	Carbohydrate (%)	60.90 ± 0.12	58.11 ± 0.25	57.68 ± 0.18	57.81 ± 0.22	57.77 ± 0.26	57.80 ± 0.27

^aPurchased from local animal feed ingredient dealer, Udaipur, Rajasthan, India
^bComposition of vitamin mineral mix (Agrimin) (quantity kg⁻¹): vitamin A- 55,00,000 IU; vitamin D3- 11,00,000 IU; vitamin B2- 2,000 mg; vitamin E- 750 mg; vitamin K- 1,000 mg; vitamin B6- 1,000 mg; vitamin B12- 6 mcg; calcium pantothenate—2,500 mg; nicotinamide—10 g; choline chloride—150 g; Mn—27,000 mg; Cu—2,000 mg; I—1,000 mg; Zn—5,000 mg; Fe—7,500 mg; Co—450 mg; L-lysine—10 g; DL—methionine- 10 g; selenium—125 mg
^cHiMedia Laboratories, Mumbai, India