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Table 2 Hepatosomatic index (HSI), visceral index (VI) and glycogen content in liver and muscle of L. rohita juvenile exposed to short term higher temperature and fed diet differing in protein level

From: Histo-architectural and histochemical changes in the liver and intestine of Labeo rohita: integrative effect of water temperature and dietary protein level

Temp.

260C

One week at 320C then at 260C

Two Way ANOVA

Variation Source

Treatment

20%CP

30%CP

40%CP

45%CP

20%CP

30%CP

40%CP

45%CP

Temp.

Protein

Inter.

HSI

 1st week

1.11a±0.1

0.91bc±0.1

0.96b±0.1

0.78cd±0.1

0.92Bbc±0.1

0.68Bdc±0.1

0.69Bdc±0.1

0.60Bc±0.1

P<0.05

P<0.05

NS

 3rd Week

1.09a±0.1

0.96bc±0.1

0.97bc±0.1

0.88c±0.1

1.05Aab±0.1

0.89Ac±0.1

0.90Ac±0.1

0.78Ad±0.1

P<0.05

P<0.05

NS

 5th week

1.10a±0.1

0.90bc±0.1

0.93b±0.1

0.85cd±0.1

1.12Aa±0.1

0.91Ab±0.1

0.90Abc±0.1

0.83Ad±0.1

NS

P<0.05

NS

VI

 1st week

4.48a±0.1

3.65b±0.2

3.26cd±0.1

3.07d±0.1

4.39a±0.1

3.46bc±0.08

3.19d±0.09

3.12d±0.07

NS

P<0.05

NS

 3rd Week

4.20a±0.1

3.62b±0.1

3.24d±0.1

3.08e±0.1

4.30a±0.1

3.44c±0.15

3.23d±0.07

3.06e±0.14

NS

P<0.05

NS

 5th week

4.29a±0.1

3.42bc±0.1

3.21cd±0.1

3.11d±0.2

4.34a±0.1

3.50b±0.10

3.18cd±0.14

3.09d±0.12

NS

P<0.05

NS

Glycogen (Liver) (mg/gm wet tissue)

 1st week

46.39a±2.85

34.65b±1.10

30.54bc±2.11

26.46cd±1.59

33.25Bb±1.39

27.22Bcd±1.45

23.16Bde±1.40

18.83Be±0.94

P<0.05

P<0.05

P<0.05

 3rd Week

46.72a±1.40

33.60bc±2.06

30.11cd±1.95

28.40cd±1.56

38.45Bb±1.89

29.68ABbc±1.10

26.81ABcd±0.94

24.73ABd±0.97

P<0.05

P<0.05

P<0.05

 5th week

46.48a±1.72

33.93bc±2.39

30.89bc±2.41

26.54c±2.31

46.17Aa±1.56

35.26Aa±2.85

30.49Abc±1.98

27.68Ac±1.91

NS

P<0.05

NS

Glycogen (Muscle) (mg/gm wet tissue)

 1st week

6.55±0.45

5.20±0.20

6.06±0.34

5.88±0.21

5.97±0.41

5.89±0.37

7.14±0.55

5.99±0.45

NS

NS

NS

 3rd Week

5.88±0.37

5.57±0.22

6.05±0.45

4.42±0.15

6.07±0.25

5.71±0.17

5.02±0.32

5.29±0.42

NS

NS

NS

 5th week

5.50±0.31

5.92±0.37

5.75±0.44

5.07±0.26

5.99±0.19

5.55±0.25

5.45±0.31

5.70±0.27

NS

NS

NS

  1. Mean values in the same row with different superscript (a, b, c, d) differ significantly (P < 0.05)
  2. Mean values in a column (1st, 3rd and 5th week) under each parameter bearing different superscript (A, B, C) differ significantly (P < 0.05)
  3. CP Crude protein, NS Not significantly different (P > 0.05)