Histo-architectural and histochemical changes in the liver and intestine of Labeo rohita: integrative effect of water temperature and dietary protein level

Kumar, Shivendra; Sahu, N. P.

doi:10.1186/s44365-024-00005-z

Table 4 Aalkaline phosphatase and acid phosphatase activity in the liver and intestine of L. rohita juvenile exposed to short term higher temperature and fed diet differing in protein level

From: Histo-architectural and histochemical changes in the liver and intestine of Labeo rohita: integrative effect of water temperature and dietary protein level

Temp	26⁰C					One week at 32⁰C then at 26⁰C			Two Way ANOVA
Temp	26⁰C					One week at 32⁰C then at 26⁰C			Variation Source
Treatment	20%CP	30%CP	40%CP	45%CP	20%CP	30%CP	40%CP	45%CP	Temp	Protein	Inter.
Alkaline phosphatase (Liver) (nano moles p-nitrophenol released/min/mg protein at 37⁰C)
1st week	15.31^a ± 1.34	11.65^b ± 0.67	8.26^c ± 0.77	9.93^bc ± 0.47	9.94^Bbc ± 0.47	8.26^Bc ± 0.28	7.74^c ± 0.43	12.07^b ± 0.81	P < 0.05	P < 0.05	P < 0.05
3rd Week	15.79^a ± 1.05	12.08^b ± 0.73	7.30^d ± 0.26	10.35^bc ± 0.35	12.75^Ab ± 0.35	9.93^ABbcd ± 0.46	8.56^cd ± 0.47	10.94^bc ± 0.54	NS	P < 0.05	NS
5th week	14.65^a ± 0.67	11.87^b ± 0.24	8.22^c ± 0.53	9.70^bc ± 0.68	14.89^Aa ± 0.62	11.93^Ab ± 0.68	9.26^c ± 0.49	10.14^bc ± 0.47	NS	P < 0.05	NS
Alkaline phosphatase (Intestine) (nano moles p-nitrophenol released/min/mg protein at 37⁰C)
1st week	7.99^f ± 0.29	13.78^e ± 1.14	17.69^d ± 0.96	16.39^de ± 1.29	26.57^Ac ± 0.55	30.86^Ab ± 0.80	37.90^Aa ± 0.92	31.13^Ab ± 0.81	P < 0.05	P < 0.05	P < 0.05
3rd Week	7.31^d ± 0.61	13.93^c ± 0.16	18.31^b ± 0.70	15.95^bc ± 0.81	14.01^Bc ± 0.91	18.19^Bb ± 0.53	22.11^Ba ± 1.01	17.50^Bb ± 0.67	P < 0.05	P < 0.05	P < 0.05
5th week	7.38^d ± 0.74	13.78^c ± 1.14	18.16^ab ± 0.68	15.92^bc ± 0.61	8.71^Cd ± 0.69	14.13^Cc ± 0.66	18.52^Ba ± 0.58	15.71^Bbc ± 0.69	NS	P < 0.05	NS
Acid phosphatase (Liver) (nano moles p-nitrophenol released/min/mg protein at 37⁰C)
1st week	3.51 ± 0.17	3.69 ± 0.29	4.13 ± 0.39	4.34 ± 0.11	3.70 ± 0.16	4.37 ± 0.24	4.33 ± 0.29	4.56 ± 0.15	NS	NS	NS
3rd Week	3.94 ± 0.24	4.42 ± 0.33	4.28 ± 0.32	4.04 ± 0.24	3.95 ± 0.19	4.79 ± 0.33	4.17 ± 0.32	4.39 ± 0.41	NS	NS	NS
5th week	4.02 ± 0.37	3.81 ± 0.25	4.37 ± 0.18	3.72 ± 0.31	4.30 ± 0.25	4.03 ± 0.12	4.87 ± 0.28	4.28 ± 0.35	NS	NS	NS
Acid phosphatase (Intestine) (nano moles p-nitrophenol released/min/mg protein at 37⁰C)
1st week	3.64^e ± 0.21	6.15^cd ± 0.40	7.71^bc ± 0.57	9.67^ab ± 0.35	4.01^de ± 0.38	6.45^c ± 0.41	8.51^abc ± 0.71	10.21^a ± 0.82	NS	P < 0.05	NS
3rd Week	3.89^f ± 0.11	5.71^de ± 0.48	8.21^bc ± 0.34	10.77^a ± 0.51	4.73^ef ± 0.50	6.71^cd ± 0.45	8.75^b ± 0.46	9.69^ab ± 0.54	NS	P < 0.05	NS
5th week	4.02^ef ± 0.27	5.85^de ± 0.47	8.85^bc ± 0.50	10.92^a ± 0.50	3.87^f ± 0.20	7.35^cd ± 0.45	9.89^ab ± 0.53	10.70^ab ± 0.32	NS	P < 0.05	NS

Mean values in the same row with different superscript (a, b, c, d) differ significantly (P < 0.05)
Mean values in a column (1st, 3rd and 5th week) under each parameter bearing different superscript (A, B, C) differ significantly (P < 0.05)
CP Crude protein, NS Not significantly different (P > 0.05)

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