Effects of substituting groundnut oil cake with Prosopis cineraria seed meal on growth, physio-metabolic, and haemato-biochemical responses of Labeo rohita

Table 2 Feed, proximate and essential amino acids composition of experimental diets

Ingredients (g kg⁻¹)	¹Treatments
	C_S	PSM₅	PSM_7.5	PSM₁₀
Fish meal^¥	50.00	50.00	50.00	50.00
Soybean meal^¥	300.00	300.00	300.00	300.00
Groundnut oil cake^¥	165.00	127.00	114.00	92.00
PSM^£	0.00	50.00	75.00	100.00
De-oiled rice bran^¥	300.00	300.00	300.00	300.00
Wheat flour^¥	80.00	55.00	50.00	50.00
Maize flour^¥	43.68	56.68	49.68	46.68
Fish oil^¥	15.00	15.00	15.00	15.00
Sunflower oil^¥	15.00	15.00	15.00	15.00
Vitamin mineral premix^©	20.00	20.00	20.00	20.00
Butylated hydroxytoluene^€	0.20	0.20	0.20	0.20
Choline chloride^€	1.00	1.00	1.00	1.00
Carboxy methyl cellulose^€	10.00	10.00	10.00	10.00
Vitamin C^€	0.12	0.12	0.12	0.12
Total	1000.00	1000.00	1000.00	1000.00
Proximate composition (g kg⁻¹ dry matter basis)
Dry matter	917.22	912.77	917.11	919.06
Crude protein	303.21	302.09	302.82	301.32
Crude fibre	82.77	84.53	85.41	86.87
Ether extract	57.72	55.56	56.30	58.29
²Nitrogen free extract	460.84	464.55	462.43	459.48
Total ash	95.46	93.28	93.04	94.04
³Gross energy (MJ kg⁻¹)	17.67	17.64	17.63	17.62
Essential amino acids (g kg⁻¹ diet)
Arginine	29.97	29.05	26.43	22.40
Histidine	7.29	7.09	5.96	4.73
Isoleucine	13.38	12.89	10.67	8.28
Leucine	22.54	22.65	19.49	16.03
Lysine	17.94	17.35	14.23	10.93
Methionine	4.46	4.55	4.02	3.43
Phenylalanine	14.73	14.53	12.38	10.00
Tryptophan	ND	ND	ND	ND
Threonine	12.01	12.06	10.39	8.60
Valine	14.83	14.57	12.34	9.90

¹C_S, 0 g kg⁻¹ PSM; PSM₅, 50 g kg⁻¹ PSM; PSM_7.5, 75 g kg⁻¹ PSM; PSM₁₀, 100 g kg⁻¹ PSM
^¥Purchased from local animal feed ingredient dealer, Mumbai, India
^£PSM- Prosopis cineraria seed meal Procured from Jaipur, Rajasthan, India
^€Procured from Himedia Pvt. Ltd., Mumbai, India
^©Composition of vitamin mineral mix (Agrimin Forte) (quantity kg⁻¹): Vitamin A- 55,00,000 IU; vitamin D3- 11,00,000 IU; vitamin B2- 2,000 mg; vitamin E- 750 mg; vitamin K- 1,000 mg; vitamin B6- 1,000 mg; vitamin B12- 6 mcg; calcium pantothenate- 2,500 mg; nicotinamide- 10 g; choline chloride- 150 g; Mn- 27,000 mg; I- 1,000 mg; Fe- 7,500 mg; Zn- 5,000 mg; Cu- 2,000 mg; Co- 450 mg; L- lysine- 10 g; DL- methionine- 10 g; selenium- 125 mg procured from Virbac, India
²Nitrogen free extract (g kg^-¹) = {1000—[Crude protein (g kg⁻¹) + Ether extract (g kg⁻¹) + Total ash (g kg⁻¹) + Crude fibre (g kg⁻¹)}
³Gross energy (GE) was calculated according to gross caloric values of using the values of 23.6, 39.5, and 17.2 kJ/g for crude protein, crude fat, and total carbohydrate, respectively (Brett, 1973)
ND, Not determined