Effects of substituting groundnut oil cake with Prosopis cineraria seed meal on growth, physio-metabolic, and haemato-biochemical responses of Labeo rohita

Table 6 Protein and carbohydrate metabolic enzyme activities of L. rohita fingerlings fed with different experimental diets with PSM for 60 days

¹Treatments	²AST		³ALT		⁴LDH		⁵MDH
¹Treatments	Liver	Muscle	Liver	Muscle	Liver	Muscle	Liver	Muscle
Cs	4.01^a	11.81^a	4.96	2.94^b	4.34^a	0.82	0.86	2.34^a
PSM₅	4.07^a	14.59^b	4.84	3.22^b	3.72^a	1.01	0.86	2.63^a
PSM_7.5	4.89^b	15.42^b	4.99	1.90^a	4.47^a	0.96	1.01	3.20^b
PSM₁₀	5.43^b	14.49^b	4.91	1.64^a	5.69^b	1.03	1.09	3.37^b
Contrast analysis
P values
Overall	0.004	0.011	0.965	< 0.001	0.006	0.302	0.175	0.004
Linear	0.013	0.024	0.893	< 0.001	0.015	0.854	0.902	0.023
Quadratic	0.009	0.015	0.349	0.006	0.011	0.278	0.206	0.009

Data expressed as Mean (n = 3); Mean values in the same column with different superscript differ significantly (P < 0.05)
¹C_S, 0 g kg⁻¹ PSM (Prosopis cineraria seed meal); PSM₅, 50 g kg⁻¹ PSM; PSM_7.5, 75 g kg⁻¹ PSM; PSM₁₀, 100 g kg⁻¹ PSM
²AST, Aspartate aminotransferase specific activity is expressed as nano moles of oxaloacetate released/min/mg protein at 37⁰C
³ALT, Alanine aminotransferase specific activity is expressed as nano moles of sodium pyruvate formed/min/mg protein at 37⁰C
⁴LDH, Lactate dehydrogenase specific activity is expressed as units/ min/mg protein at 37⁰C
⁵MDH, Malate dehydrogenase specific activity is expressed as units/ min/ mg protein at 37⁰C